Journal: Journal of medicinal chemistry

Article Title: Discovery of 6-Phenylhexanamide Derivatives as Potent Stereoselective Mitofusin Activators for the Treatment of Mitochondrial Diseases

doi: 10.1021/acs.jmedchem.0c00366

Figure Lengend Snippet: Diastereomer-selective correction of mitochondrial fragmentation and depolarization in Mfn knockout cells for 13. A and B. Dose-response curves for 13 stereoisomers increasing mitochondrial aspect ratio in cells expressing only Mfn2 (A) or only Mfn1 (B). Prototype mitofusin activator 2 data are shown for comparison. C. Effects of compounds on mitochondrial inner membrane polarization in Mfn1 knockout (KO; top) and Mfn2 KO (bottom) cells. D. Representative confocal imaging of mitochondrial morphology and polarization status in Mfn2 null MEFs treated with different compounds (1 μM, 24 h). Green mitochondria are depolarized and have damaged respiratory function. Scale bars are 10 μm. MT Green (mitotracker green), TMRE (tetramethylrhodamine ethyl ester, red) and nuclear Hoescht (blue) were used for staining. Data are means ± SEM of three independent experiments.

Article Snippet: Functional evaluation of mitofusin agonist fusogenicity was performed in MFN1- or MFN2-deficient MEFs purchased from ATCC (Cat #: CRL-2992, CRL-2993) and cultured at 37°C, 5% CO 2 -95% air in Dulbecco’s minimal essential medium (DMEM) containing glucose (4.5 g/l) with 10% (v/v) fetal bovine serum (FBS; Gibco, 26140–079), 1x nonessential amino-acids (Gibco, 11130051), 2 mM L-glutamine (Corning, 34717007), and 1% (v/v) penicillin/streptomycin (Gibco, 15140–122).

Techniques: Knock-Out, Expressing, Imaging, Staining

Journal: Journal of Functional Biomaterials

Article Title: Comparison of Key Properties of Ag-TiO 2 and Hydroxyapatite-Ag-TiO 2 Coatings on NiTi SMA

doi: 10.3390/jfb15090264

Figure Lengend Snippet: Cytotoxicity of the Ag-TiO 2 ( a ) and HAp-Ag-TiO 2 ( b ) against fibroblast (NHDF) and osteoblast (HOB) cells. Microscope imaging of A—NHDF cell line grown on the coatings, B—NHDF cell line grown on the NiTi substrate, C—HOB cell line grown on the coatings, and D—HOB cell line grown on the NiTi substrate for Ag-TiO 2 ( c ) and HAp-Ag-TiO 2 ( d ) coatings.

Article Snippet: To perform biocompatibility, tests of the normal human dermal fibroblast (NHDF) and the human osteoblasts (HOB), obtained from PromoCell (Heidelberg, Germany) were used.

Techniques: Microscopy, Imaging

Journal: Molecular Therapy Oncolytics

Article Title: Dependency of EGFR activation in vanadium-based sensitization to oncolytic virotherapy

doi: 10.1016/j.omto.2022.04.004

Figure Lengend Snippet: Gefitinib reduces the effects of vanadium-VSVΔ51 combination therapy in vivo (A and B) CT26WT tumor cores were obtained from implanted Balb/c mice and treated ex vivo with vanadate (300 μM) ± gefitinib (50 μM). Cores were infected 4 h later with 3e4 plaque forming units (pfu) of VSVΔ51-GFP per core. (A) Fluorescence images were taken 24 hpi; scale bar, 1000 μm. (B) Supernatant was collected 48 hpi and viral titer was assessed by plaque assay (n = 3, mean ± SEM; ∗∗p < 0.01 by one-way ANOVA). (C and D) Balb/c mice were implanted subcutaneously with CT26WT and allowed to progress to 100 mm 3 . Mice were then injected intratumorally with vanadyl sulfate (50 mg/kg) ± gefitinib (100 mg/kg) for 4 h. Mice were then injected intratumorally with VSVΔ51-FLuc (1e8 pfu/tumor). At 24 hpi, mice were imaged using a live imaging system (IVIS) for luminescence activity. (C) Absolute luminescence was log-transformed and graphed (n = 8–10, mean ± SEM; ∗p < 0.05 by one-way ANOVA). (D) Representative luminescence images are shown.

Article Snippet: The 786-0 (human male renal cell adenocarcinoma, cat. CRL-1932), CT26WT (murine colon carcinoma, cat. CRL-2638), and Vero (African green monkey kidney cells, cat. CCL-81) were acquired from the American Type Culture Collection.

Techniques: In Vivo, Ex Vivo, Infection, Fluorescence, Plaque Assay, Injection, Imaging, Activity Assay, Transformation Assay

Journal: Cell Reports

Article Title: Notch Signaling Mediates Secondary Senescence

doi: 10.1016/j.celrep.2019.03.104

Figure Lengend Snippet:

Article Snippet: IMR90 normal human diploid fibroblasts , ATTC , ATCC Cat# CRL-7931, RRID:CVCL_0347.

Techniques: Plasmid Preparation, Recombinant, cDNA Synthesis, Imaging, Multiplex Assay, Software

Journal: Scientific Reports

Article Title: Hypoxia induces pulmonary fibroblast proliferation through NFAT signaling

doi: 10.1038/s41598-018-21073-x

Figure Lengend Snippet: Hypoxia induces pulmonary fibroblast proliferation. Normal human pulmonary fibroblasts (HPFs) and LL29 IPF fibroblasts were exposed to normoxia (21% O 2 ) or hypoxia (5% or 1% O 2 ) for 2–6 days. ( A ) Bright field imaging. Scale bar: 100 µm. ( B ) Cell count. ( C , D ) Cell proliferation by BrdU assay. HPF and LL29 cells were incubated with BrdU for 3 hrs. Data were normalized to normoxia. The absorbance for BrdU at normoxia was 0.08 ± 0.002 (day 3) and 0.09 ± 0.009 (day 6) compared to 0.09 ± 0.004 (day 3) and 0.10 ± 0.006 (day 6) for HPF and LL29 cells, respectively. ( E , F ) Cell viability by MTT assay. The absorbance for MTT at normoxia was 0.23 ± 0.0796 (day 3) and 0.30 ± 0.091 (day 6) compared to 0.07 ± 0.016 (day 3) and 0.28 ± 0.097 (day 6) for HPF and LL29 cells, respectively. ( G ) Additional normal human pulmonary fibroblasts [HPF (with F12K medium), CCD-13Lu, CCD-19Lu, HPF153 and HLF154] and IPF fibroblasts (LL97A, IPF12 and IPF14) were subjected to normoxia and hypoxia (1% O 2 ) for 3 days, and cell proliferation assessed by BrdU assay. Cells were incubated with BrdU for 12 hrs. The absorbance values for BrdU at normoxia were 0.15 ± 0.006 (HPF with F12K medium), 0.09 ± 0.004 (CCD-13Lu), 0.18 ± 0.008 (CCD-19Lu), 0.28 ± 0.003 (HLF153), 0.14 ± 0.008 (HLF154), 0.11 ± 0.004 (LL97A), 0.05 ± 0.003 (IPF12) and 0.10 ± 0.01 (IPF14); values represent means ± SE. *p < 0.05, **p < 0.01, ***p < 0.001 vs. normoxia. n = 3 independent experiments.

Article Snippet: IPF fibroblasts LL29 and LL97A were isolated from a 26-year-old female Caucasian patient and a 48-year-old male Caucasian patient, respectively, and were purchased from American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Imaging, Cell Counting, BrdU Staining, Incubation, MTT Assay

Journal: Stem Cells

Article Title: Three‐dimensional migration of human amniotic fluid stem cells involves mesenchymal and amoeboid modes and is regulated by mTORC1

doi: 10.1002/stem.3441

Figure Lengend Snippet: Human amniotic fluid stem cell (hAFSC) characterization for cell morphology and cell size. A,B, Phase contrast imaging for the evaluation of cell morphology in c‐Kit‐selected hAFSC lines (A) and low passage human amniotic fluid cells obtained via amniocentesis performed for routine prenatal diagnosis (B). The bottom panels represent a twofold magnification of the corresponding image section marked with a rectangle. C, Volume‐based cell size distribution of logarithmically growing hAFSCs ( AFS ). For comparison, human amniotic fluid cells ( AF ) and primary as well as immortalized/transformed cells of mesenchymal origin were co‐analysed. The latter include fetal/neonatal primary human lung and foreskin fibroblasts (IMR‐90 and CCD‐1079Sk, HFF‐1, respectively), adult primary cardiac fibroblasts and chondrocytes (HCF and HCH, respectively), mouse embryonic fibroblasts (NIH/3T3) and human fibrosarcoma cells (HT1080). “AFS‐all” represents the average of all values obtained for the hAFSC lines shown

Article Snippet: Amniotic fluid was provided by Gabor Joo (Semmelweis University Medical School, Budapest, Hungary); IMR‐90, primary human lung fibroblasts (ATCC, US, #CCL‐186); CCD‐1079Sk, primary human foreskin fibroblasts (ATCC, #CRL‐2097); HFF‐1, primary human foreskin fibroblasts (ATCC, #SCRC‐1041); HCF, primary human cardiac fibroblasts isolated from the ventricle of an adult heart (Promocell, Germany, #C‐12375); HCH, primary human chondrocytes isolated from normal human articular cartilage from the femoral head (Promocell, #C‐12710); NIH/3T3, immortalized mouse embryonic fibroblasts (ATCC, #CRL‐1658); HT1080, human fibrosarcoma cells (ATCC, #CCL‐121); MCF7, human mammary carcinoma cells (ATCC, #HTB‐22); MDA‐MB‐231, human mammary carcinoma cells (ATCC, #HTB‐26); HCC‐1.2 and HCC‐1.1, human hepatocellular carcinoma cells ; HUVEC, primary human umbilical vein endothelial cells (Promocell, #C‐12200); Hep G2, human hepatocellular carcinom cells (ATCC, #HB‐8065); and ARPE‐19, human retinal pigmented epithelium cells (ATCC, #CRL‐2302).

Techniques: Imaging, Biomarker Discovery, Comparison, Transformation Assay

Journal: Stem Cells

Article Title: Three‐dimensional migration of human amniotic fluid stem cells involves mesenchymal and amoeboid modes and is regulated by mTORC1

doi: 10.1002/stem.3441

Figure Lengend Snippet: The epithelial‐mesenchymal transition (EMT)‐related marker profile of human amniotic fluid stem cells (hAFSCs). A, Immunoblotting for the detection of EMT‐related marker proteins in epithelial ( ep ) and mesenchymal ( ms ) cells of fibroblast (CCD‐1079Sk, HT1080), mammary (MCF7, MDA‐MB‐231), and hepatic (HCC‐1.2, HCC‐1.1) origins. l.e ., long exposure; pro , pro‐form; act , active form. B, Immunoblotting for the analysis of EMT‐related marker proteins in hAFSCs. Epithelial and mesenchymal cells were co‐analysed as a reference. For data presented in the left panel, the same lysates were separated on two different gels ( Blot#1 and Blot#2 ). l.e ., long exposure; pro , pro‐form; act , active form; FL , full‐length; CL , cleaved. C, Gelatin zymography for the detection of secreted MMPs in hAFSCs. Recombinant human MMP2 and MMP9, and cells of epithelial and mesenchymal origin were co‐analyzed as controls. For better visualization of results, a color‐inverted version of the long exposure was included ( l.e. INV ). D, Summary of results obtained in B and C including the hAFSC passage numbers and the quantification results for inactive, cleaved vimentin

Article Snippet: Amniotic fluid was provided by Gabor Joo (Semmelweis University Medical School, Budapest, Hungary); IMR‐90, primary human lung fibroblasts (ATCC, US, #CCL‐186); CCD‐1079Sk, primary human foreskin fibroblasts (ATCC, #CRL‐2097); HFF‐1, primary human foreskin fibroblasts (ATCC, #SCRC‐1041); HCF, primary human cardiac fibroblasts isolated from the ventricle of an adult heart (Promocell, Germany, #C‐12375); HCH, primary human chondrocytes isolated from normal human articular cartilage from the femoral head (Promocell, #C‐12710); NIH/3T3, immortalized mouse embryonic fibroblasts (ATCC, #CRL‐1658); HT1080, human fibrosarcoma cells (ATCC, #CCL‐121); MCF7, human mammary carcinoma cells (ATCC, #HTB‐22); MDA‐MB‐231, human mammary carcinoma cells (ATCC, #HTB‐26); HCC‐1.2 and HCC‐1.1, human hepatocellular carcinoma cells ; HUVEC, primary human umbilical vein endothelial cells (Promocell, #C‐12200); Hep G2, human hepatocellular carcinom cells (ATCC, #HB‐8065); and ARPE‐19, human retinal pigmented epithelium cells (ATCC, #CRL‐2302).

Techniques: Marker, Western Blot, Zymography, Recombinant

Journal: Neuro-Oncology Advances

Article Title: The brain-penetrant cell-cycle inhibitor p28 sensitizes brain metastases to DNA-damaging agents

doi: 10.1093/noajnl/vdad042

Figure Lengend Snippet: p28 crosses the BBB and preferentially localizes to Brain metastases (BMs). (A) Confocal images of the penetration of normal and cancer cells by p28. Human cancer cell lines (MDA-231BR, BCA-1, Mel-7, and A549) and normal cells (MCF-10A and fibroblasts) were cultured with Alexa Fluor 568-labeled p28 at 37°C for 2 hours, and images were obtained by confocal microscopy. Red, p28; blue, DAPI (nucleus). (B) MDA-231BR brain-specific metastatic triple-negative breast cancer, BCA-1 breast cancer, Mel-7 melanoma, or A549 lung cancer cells were injected into the left cardiac ventricle of athymic mice. ICG-labeled p28 was intravenously injected into the mice. Near-infrared fluorescence imaging of the ICG-p28 signal (gray) in coronal brain sections (yellow dotted line on the anterior-dorsal view) of mice injected with MDA-231BR, BCA-1 or Mel-7 cells or in the anterior-dorsal view of the brain of mice injected with A549 cells. H&E staining of brain sections confirmed the presence of BMs (Tu).

Article Snippet: A549 human lung cancer cells, MCF10A, and fibroblasts were purchased from American Type Culture Collection.

Techniques: Cell Culture, Labeling, Confocal Microscopy, Injection, Fluorescence, Imaging, Staining

Journal: Redox Biology

Article Title: Identification of tyrosine brominated extracellular matrix proteins in normal and fibrotic lung tissues

doi: 10.1016/j.redox.2024.103102

Figure Lengend Snippet: PXDN and laminin expression are modulated in human lung fibroblasts (HLF) and bone-marrow-derived macrophages (BMDM) upon TGF-β1 stimulation. (A – C) HLFs were treated 5 ng/mL TGF-β1 for 48 h, and analyzed for LAMA1 (A) or PXDN (B) mRNA or PXDN protein expression (C). (D – G) BMDM from C57BL6/NJ mice were treated 5 ng/mL TGF-β1 for 48 h, and analyzed for Pxdn (D) , Lama1 (E) , or Lamb1 (F) mRNA, or Laminin α/β1 protein expression by immunofluorescence imaging (G). Scale bar: 50 μm. Values are the mean of at least three independent biological replicates ± SEM. Differences among groups were evaluated by Student's T-test.

Article Snippet: HLF (ATCC PCS-201-013) were cultured in fibroblast basal medium (ATCC PCS-201-030) with fibroblast growth kit-low serum (ATCC PCS-201-041) at 37 °C in a 5% CO 2 atmosphere.

Techniques: Expressing, Derivative Assay, Immunofluorescence, Imaging

Journal: Oncotarget

Article Title: SRF promotes gastric cancer metastasis through stromal fibroblasts in an SDF1-CXCR4-dependent manner

doi: 10.18632/oncotarget.10024

Figure Lengend Snippet: A. SRF and αSMA expression levels in CCD18Co cells stably transfected with the SRF-pTRIPZ or shSRF-LV3 vector were determined by western blotting. B. The transwell model containing an insert used to detect the effect of various fibroblast-conditioned medium on the migration of cultured cancer cells. C. The migration of MKN45 cells cultured for 12 hrs with conditioned medium from CCD18Co cells with upregulation or downregulation of SRF expression were determined by the transwell assay as described in (B) . The individual cell numbers in 3 wells were used to calculate the average value. D. The migration of MKN45 cells cultured with different conditioned medium was determined using the wound-healing assay. The values from 6 wells were used to calculate the mean and SD at each time point for each treatment.

Article Snippet: The mouse NIH3T3, human breast cancer cell MDA-MB-435S, and fibroblast CCD18Co cell lines were purchased from ATCC.

Techniques: Expressing, Stable Transfection, Transfection, Plasmid Preparation, Western Blot, Migration, Cell Culture, Transwell Assay, Wound Healing Assay

Journal: Oncotarget

Article Title: SRF promotes gastric cancer metastasis through stromal fibroblasts in an SDF1-CXCR4-dependent manner

doi: 10.18632/oncotarget.10024

Figure Lengend Snippet: A and B. The average lung weight and number of metastatic nodules on the lung surface were recorded 29 and 21 days after the co-injection of MKN45 cells, and either SRF -overexpressing CCD18Co cells or CCD18Co cells treated with shSRF, respectively. C and D. The tumor xenografts were shown after 16 and 15 days following the co-injection of MKN45 cells and either SRF- overexpressing CCD18Co cells or CCD18Co cells treated with shSRF, respectively. The hematoxylin and eosin (H&E)-stained tumor tissues were also shown. Black bar: 200 μm. The data represent the mean ± SD.

Article Snippet: The mouse NIH3T3, human breast cancer cell MDA-MB-435S, and fibroblast CCD18Co cell lines were purchased from ATCC.

Techniques: Injection, Staining

Journal: Oncotarget

Article Title: SRF promotes gastric cancer metastasis through stromal fibroblasts in an SDF1-CXCR4-dependent manner

doi: 10.18632/oncotarget.10024

Figure Lengend Snippet: A and B. The results from qRT-PCR experiments shows the mRNA levels of SRF , αSMA , SDF1 , MMP2 , and TGF-β in CCD18Co cells stably infected with the SRF or shSRF pTRIPZ vectors 24 hrs after doxycycline induction; C. The concentration of SDF1 in the CCD18Co conditioned medium was determined by ELISA. D. qRT-PCR revealed a significant correlation between SRF and SDF1 mRNA levels in GC tissues. The data represent the mean ± SD.

Article Snippet: The mouse NIH3T3, human breast cancer cell MDA-MB-435S, and fibroblast CCD18Co cell lines were purchased from ATCC.

Techniques: Quantitative RT-PCR, Stable Transfection, Infection, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Oncotarget

Article Title: SRF promotes gastric cancer metastasis through stromal fibroblasts in an SDF1-CXCR4-dependent manner

doi: 10.18632/oncotarget.10024

Figure Lengend Snippet: The relative wound density was recorded over time using the IncuCyte ZOOM™ live-cell imaging platform. The migration capacity of MKN45 cells cultured in conditioned medium from CCD18Co cells stably transfected with the SRF or control vector was detected over time. A. MKN45 cells were cultured in the conditioned medium with or without an anti-SDF1 antibody (SDF1ab). B. MKN45 cells were cultured in conditioned medium with or without AMD3100. Each value in the right charts is the average value of 6 wells. The data represent the mean ± SD.

Article Snippet: The mouse NIH3T3, human breast cancer cell MDA-MB-435S, and fibroblast CCD18Co cell lines were purchased from ATCC.

Techniques: Live Cell Imaging, Migration, Cell Culture, Stable Transfection, Transfection, Control, Plasmid Preparation

Journal: Oncotarget

Article Title: SRF promotes gastric cancer metastasis through stromal fibroblasts in an SDF1-CXCR4-dependent manner

doi: 10.18632/oncotarget.10024

Figure Lengend Snippet: A and B. The migration capacity of MKN45 cells cultured in the conditioned medium from CCD18Co cells stably transfected with the SRF or control vector and treated with anti-SDF1 antibody (SDF1ab) or AMD3100. The images were captured 12 hrs after co-culture. The data represent the mean ± SD (n=3).

Article Snippet: The mouse NIH3T3, human breast cancer cell MDA-MB-435S, and fibroblast CCD18Co cell lines were purchased from ATCC.

Techniques: Migration, Cell Culture, Stable Transfection, Transfection, Control, Plasmid Preparation, Co-Culture Assay

Journal: ACS Nano

Article Title: Illuminating the Underlying Mechanism of Intracellular Optoelectronic Modulation Using Silicon Nanowires

doi: 10.1021/acsnano.5c11146

Figure Lengend Snippet: The effect of photoanodic, photocathodic, and pure photothermal modulation on intracellular calcium dynamics: (A) Fluorescence images of stimulated normal human dermal fibroblasts (NHDF) cells loaded with a calcium indicator (green) and containing silicon nanowires (SiNWs, yellow). The top panels show NHDF-p–i–n hybrids, the middle panels NHDF-n–i–p hybrids, and the bottom panels NHDF-i–i–i hybrids. Red arrows indicate the stimulated SiNW using a 640 nm laser, for 3 ms, at 1.6 mW. Scale bars are 20 μm. (B) d F / F image captured at 13.5 s after stimulation, with a white arrow marking the line used to generate the kymograph shown in panel (C). Scale bars are 20 μm. (C) Representative kymographs of d F / F traces used to quantify intracellular calcium flux in the stimulated cells shown in (A). The top and middle panels show high calcium propagation rate following stimulation of p–i–n and n–i–p SiNWs, respectively. In contrast, the bottom panel shows slower propagation in i–i–i SiNW-stimulated cells. (D) Generated binary mask using MATLAB, with a white dashed line representing the slope of calcium propagation. Linear regression was applied to estimate the calcium flux velocity ( v = d x /d t ). (E) Box plots showing intracellular calcium propagation velocities (μm/s), derived from kymographs in (C) for cells stimulated with a 640 nm laser (3 ms, 1.6 mW). Data represent measurements from 12 samples. * p < 0.05, ** p < 0.01.

Article Snippet: Normal human dermal fibroblasts (NHDF) (PCS-201-010) were purchased from ATCC.

Techniques: Fluorescence, Generated, Derivative Assay

Journal: ACS Nano

Article Title: Illuminating the Underlying Mechanism of Intracellular Optoelectronic Modulation Using Silicon Nanowires

doi: 10.1021/acsnano.5c11146

Figure Lengend Snippet: Intracellular optoelectronic modulation and calcium response characterization. (A) Left-confocal imaging of a normal human dermal fibroblast (NHDF) cell with an internalized n–i–p silicon nanowire (SiNW) (reflection, yellow) and loaded with a calcium indicator (fluo-4, green), scale bar is 20 μm. The red arrow highlights the stimulated SiNW inside the cell, and a cross-sectional view of the z -stack along the white arrow (right) verifies that the SiNW is internalized, scale bar is 3 μm. (B) Illustration of intracellular stimulation via optical modulation of the SiNW. (C,D) Fluorescent (C) and derived d F / F (D) images of the stimulated cell (using 640 nm laser, for 3 ms at 1.6 mW) captured at different time points: 54 ms before stimulation, and 68 ms, 2.154 s, and 13.41 s after stimulation. The white arrow in (D) (right image) indicates the line used for cross-sectional kymograph analysis (see panel (F)). Scale bars are 20 μm. (E) Heatmap of the cell showing d F / F analysis from a time-series data set, illustrating calcium propagation using time color code, scale bar is 20 μm. (F) Kymograph of d F / F presenting the spatial and temporal dynamics of calcium flux originating from the stimulated SiNW. (G). d F / F plot of calcium response following stimulation of the n–i–p SiNW with a 1.6 mW laser at t = 2.7 s.

Article Snippet: Normal human dermal fibroblasts (NHDF) (PCS-201-010) were purchased from ATCC.

Techniques: Imaging, Derivative Assay

Journal: ACS Nano

Article Title: Illuminating the Underlying Mechanism of Intracellular Optoelectronic Modulation Using Silicon Nanowires

doi: 10.1021/acsnano.5c11146

Figure Lengend Snippet: Optically induced calcium transients in normal human dermal fibroblasts are driven by internal calcium sources. (A) Left image, confocal image of an untreated normal human dermal fibroblast (NHDF)-p–i–n hybrid (top) or NHDF treated with 5 μM thapsigargin (bottom), loaded with calcium indicator. Right panels: d F / F images of the stimulated cells captured at multiple time points: 54 ms before stimulation, and 54 ms, and 13.5 s after stimulation (640 nm laser, 3 ms, 2.2 mW). Scale bars are 20 μm. (B) Box plots showing the maximum d F / F values of Fluo-4 AM fluorescence after stimulation of NHDF-p–i–n hybrids with a 640 nm laser (3 ms, 2.2 mW). n > 6. *** p < 0.001.

Article Snippet: Normal human dermal fibroblasts (NHDF) (PCS-201-010) were purchased from ATCC.

Techniques: Fluorescence

Journal: ACS Nano

Article Title: Illuminating the Underlying Mechanism of Intracellular Optoelectronic Modulation Using Silicon Nanowires

doi: 10.1021/acsnano.5c11146

Figure Lengend Snippet: Photoelectrochemical induction of reactive oxygen species (ROS) release in normal human dermal fibroblasts (NHDF). (A) d F / F images of NHDF cells with internalized silicon nanowires (SiNWs), stained with the fluorescent ROS indicator CellROX Orange, acquired at t = 58 s. Cells were stained with CellROX and imaged for ∼1 min without stimulation. Scale bars are 20 μm. (B) d F / F images of NHDF cells with internalized SiNWs, stained with the fluorescent ROS indicator CellROX Orange. Cells were imaged for ∼1 min and optically stimulated during imaging at t = 5.7 s (640 nm, 3 ms, and 1.6 mW), while presented images were acquired at t = 58 s. (A,B) Left to right tilescells without SiNWs, NHDF-p–i–n hybrids, NHDF-n–i–p hybrids, and NHDF-i–i–i hybrids. Cells without SiNWs and cells with p–i–n and i–i–i SiNWs showed minimal d F / F changes, while n–i–p hybrids exhibited strong d F / F increases. The graph on the right shows d F / F trends for each group. Scale bars are 20 μm. (C) Leftwithout stimulation: Box plots showing the maximum d F / F values during the 1 min recording without stimulation ( n > 30). Rightwith stimulation: Box plots displaying the maximum d F / F values in response to optical stimulation (1.6 mW, 640 nm) at t = 2.7 s, during a 1 min recording ( n > 8). (D) Box plots displaying the RFU values indicating H 2 O 2 generation in response to optical stimulation of SiNWs in suspension. SiNWs were stimulated using red and lime LEDs at 2.5 Hz for 1 h at 0.25 W. For (C,D), the statistical tests were two-way ANOVA, * p < 0.05, *** p < 0.001, and **** p < 0.0001.

Article Snippet: Normal human dermal fibroblasts (NHDF) (PCS-201-010) were purchased from ATCC.

Techniques: Staining, Imaging, Suspension

Journal: ACS Nano

Article Title: Illuminating the Underlying Mechanism of Intracellular Optoelectronic Modulation Using Silicon Nanowires

doi: 10.1021/acsnano.5c11146

Figure Lengend Snippet: Investigation of calcium signaling induced by non-Faradaic p–i–n silicon nanowires (SiNWs) in normal human dermal fibroblasts (NHDFs). (A) Confocal images of NHDF-p–i–n hybrids loaded with the calcium indicator Fluo-4 AM. The panels show untreated cells, cells treated with 20 μM 2-APB (2APB), 40 μM 2-APB, or 20 μM dantrolene sodium (DS). Scale bars are 20 μm. (B) Corresponding d F / F images of the same cells shown in (A), captured at 13.5 s after optical stimulation of the internalized p–i–n SiNWs using a 640 nm laser (8 mW, 3 ms). Scale bars are 20 μm. (C) Box plots displaying the maximum Δ F / F values in response to the stimulation (*** p < 0.001), and (* p < 0.05) n > 7.

Article Snippet: Normal human dermal fibroblasts (NHDF) (PCS-201-010) were purchased from ATCC.

Techniques: